The microDUO is a high-through- put screening (HTS) compatible microplate that enables cellular co-culture of a variety of tissue models without the need of inserts or membranes. This elegant solu- tion is technically simple and seamlessly integrates with ex- isting HTS robotic infrastruc- ture. Our 384-well format consist of 192 co-culture arrays, unleashing a powerful tool with utility in drug screening and chemical testing.